| Nari Samir | 6 | Human |
| Narrator | 1 | Human |
| Narrator | 1 | Human |
| Nascent Snallygaster | 14 | Snallygaster |
| Natalie Ingesson | 6 | Human |
| National Guard Protectron | 14 | Protectron |
| Naughty Showman | 1 | Ghoul |
| Necrotic Sheepsquatch | 100 | Sheepsquatch |
| Needles | 66 | Human |
| Nellie Wright | 1 | Human |
| Nightstalker | 100 | Wendigo |
| Noodles | 1 | Mole Rat |
| Noxious Fog Crawler | 51 | Fog Crawler |
| Noxious Sheepsquatch | 80 | Sheepsquatch |
| NPC1 | 1 | Ghoul |
| NPC2 | 1 | Human |
| Nuclear Don | 66 | Human |
| Nuka-Quantron | 3 | Protectron |
| Nukatron | 3 | Protectron |
| NW Machinegun Turret | 16 | Tripod Turret |
| Observer Errol | 66 | Human |
| Observer Johanna | 66 | Human |
| Observer Marlon | 66 | Human |
| Officer Cecil | 1 | Human |
| Officer Klein | 1 | Human |
| Officer Sidney | 1 | Human |
| Officer Walton | 1 | Human |
| Ogua | 0.1 | Ogua |
| Old Man Remus | 1 | Human |
| Older Woman | 1 | Human |
| Ollie | 1 | Human |
| Operations Director | 50 | Robobrain |
| Opossum | 1 | Opossum |
| Opossum | 1 | Opossum |
| Optimized Virus Scanner | 38 | EyeBot |
| Orlando | 66 | Human |
| Ornithologist | 1 | Human |
| Oscar Gonzalez | 66 | Human |
| Out of Tune Musician | 100 | Ghoul |
| Outpost Settler | 66 | Human |
| Outpost Settler | 66 | Human |
| Overcharged Virus Scanner | 20 | EyeBot |
| Overgrown Elder | 10 | Overgrown Tank |
| Overgrown Elder | 10 | Overgrown Tank |
| Overgrown Elder | 1 | Overgrown Tank |
| Overgrown Elder Bruiser | 10 | Overgrown Tank |
| Overgrown Elder Bruiser | 66 | Overgrown Tank |
| Overgrown Elder Bruiser | 50 | Overgrown Tank |
| Overgrown Elder Demolisher | 10 | Overgrown Tank |
| Overgrown Elder Demolisher | 66 | Overgrown Tank |